product description

This product is a special reagent for Real Time PCR using the probe method. It contains an antibody-blocked hot-start enzyme, which can effectively inhibit the non-specific annealing of primers or non-specific amplification caused by primer dimers under low temperature conditions, and improve the efficiency of the amplification reaction. specificity. This product has high amplification efficiency and detection sensitivity, can obtain a good standard curve in a wide quantitative area, and can accurately quantify. Rad and Roche et al.

Product Specifications

Product Features

High reaction specificity: The optimized system can avoid non-specific amplification to the greatest extent and has high detection specificity.
     Sensitive and fast response: The core enzyme has stable performance, can achieve rapid amplification, high detection sensitivity, and short reaction time.
     Strong anti -interference ability: With strong anti-interference ability, even complex samples can obtain ideal results.

Experimental example

       Example 1  EH002 Serial Dilution Sample Linearity Test Results

Serially diluted samples were amplified using SANSHIBIO 2x Probe qPCR Mix (EH002) . The experimental results show that EH002 has good and stable amplification performance, and
the linear coefficient of the amplification CH value of the seven sample gradients is >0.99.

            Example 2  EH002 Sample Repeatability Test Results

Samples were replicated using SANSHIBIO 2x Probe qPCR Mix (EH002)
. The experimental results show that EH002 has good and stable amplification performance, and the coefficient of variation CV of the amplification C1 value of the same
sample is <3%.