product description

This product is a special reagent for Real Time PCR using SYBR Green I dye chimeric fluorescence method. It contains antibody-blocked hot-start enzyme, which can effectively inhibit the non-specific annealing of primers or non-specific amplification caused by primer dimers under low temperature conditions. Improve the specificity of the amplification reaction. This product has the characteristics of high amplification efficiency, strong detection specificity and sensitivity, good stability, and easy operation and use. Compatible with PCR machines such as Applied Biosystems, Eppendorf. Bio-Rad and Roche, etc.

Product Specifications

Features

High specificity: Antibody-blocking hot-start enzyme with high blocking efficiency is used, no primer dimers appear, and it has high specificity and high reproducibility.

High stability: A unique stabilizer is added to the reaction buffer, and the performance remains unchanged after incubation at 37°C for more than one week. Easy to transport and melts quickly at room temperature.

Wide range of applications: A unique enhancer is added to the reaction buffer, which has a lower melting temperature, which is beneficial to the amplification of sequences with high GC content and has a wider linear range.

Experimental example

          Example 1  EH003 Sample Linearity Test Results

     Using SANSHIBIO 2x SYBR Green qPCR Mix 

(EH003), serially diluted samples were amplified. The experimental
results show that EH003 has good and stable amplification performance, and
the linear coefficient of the amplification Ct value of the seven sample gradients is > 0.99.

      Example 2  EH003 Sample Repeatability Test Results

Using SANSHIBIO 2x SYBR Green qPCR Mix 

(EH003), the same-concentration samples were amplified. The experimental results
show that EH003 has good and stable amplification performance, and
the coefficient of variation CV of the amplified Ct value of the same sample concentration is CV <3%.

      Example 3  EH003 Amplification Melting Curve Test Results

Using SANSHIBIO 2x SYBR Green qPCR Mix 

(EH003), amplification and melting curve
analysis . The experimental results show that EH003 has good and stable
amplification performance, good uniformity of melting temperature, and no non-primers such as primer dimers.

specific amplification.