product description

This product is an antibody-blocked hot-start enzyme, which can inhibit the non-specific amplification caused by the non-specific annealing of primers or primer dimers under low temperature conditions, and is suitable for Hot Start PCR. Since the antibody has been inactivated in the initial DNA denaturation step of the PCR reaction, it can be used under normal PCR reaction conditions without special inactivation treatment. The PCR product amplified with this product has an "A" base attached to the 3' end, so it can be directly cloned into T-Vector or cloned into blunt-end vector after end blunting and phosphorylation.

Product Specifications

ProduceProduct use

Hot Start PCR

DNA sequencing

Experimental example

                Example 1   EH001 Sample Linearity Test Results

Serially diluted samples were amplified using SANSHIBIO Taq Hot-Start DNA Polymerase (EH001). The experimental results show that EH001 has good and stable amplification performance, and the linear coefficient of the amplification Ct value of the seven sample gradients is > 0.99.

                    Example 2   EH001 Sample Repeatability Test Results

Samples at the same concentration were amplified using SANSHIBIO Taq Hot-Start DNA Polymerase (EH001). The experimental results show that EH001 has good and stable amplification performance, and the coefficient of variation CV of the amplified Ct value of the same sample concentration is <3%.