Real-time fluorescence quantitative PCR instrument product advantages

*Reaction plate setup guide, making experimental design very simple, even for complex multiplex quantitative experiments.

*Real-time monitoring of amplification reaction curve

* Automatically set the fluorescence baseline and automatically calculate the fluorescence threshold to greatly simplify the analysis of experimental results

*Absolute quantification of target genes using standard curve

*The software for relative quantification of gene expression provides a powerful result analysis display, which can automatically analyze the data of up to 10 96-well plate gene expression experiments at the same time, and automatically remove irrelevant data. This software is an option

*Automatic SNP typing software outputs typing results with intuitive graphs and automatically performs typing quality scoring

*Dissociation curve data collection is simple and easy to observe, and the dissociation curve data can be observed while the instrument is running

*It is very convenient to calibrate the corresponding sample well position when observing the real-time amplification curve

*Light source usage time monitoring and instrument system self-diagnosis program

The main applications of real-time quantitative PCR:

Real-time quantitative PCR has a wide range of applications, including gene expression analysis based on relative quantitative analysis, absolute quantitative analysis of pathogens based on standard curve, qualitative SNP genotyping analysis of nucleic acid sequences after PCR amplification, and positive internal control-based positive control. / Negative result judgment

fast thermal cycling

The standard mode of the 7500 real-time fluorescence quantitative PCR system uses a reaction volume of 25-100 microliters, and can use a 96-well plate or a single tube/8 strips for experimental reactions. In addition, the 7500 can also be upgraded to a rapid thermal cycle sample base, which is an option and is installed by AB engineers. The rapid thermal cycle sample base uses a new 96-well reaction plate, which has a small space above the liquid in the tube and can support experiments with a reaction volume of 5-30 microliters. Using the upgraded 7500 sample base, the new 96-well reaction plate and the new rapid thermal cycle quantitative PCR premix reagent, the real-time quantitative PCR experiment can be completed within 40 minutes, and the experimental effect is exactly the same as the standard 2-hour mode. .