1. Laboratory air-conditioning ventilation system design and pressure control

The PCR laboratory does not have strict purification requirements, but in order to avoid the possibility of cross-contamination between various experimental areas, it is advisable to adopt the air organization form of full delivery and full exhaust. At the same time, the ratio of supply and exhaust air should be strictly controlled to ensure the pressure requirements of each experimental area.

2. Pollution prevention and control  

A central issue in PCR laboratory design is how to avoid contamination. In practical work, the following types of contamination are common: contamination of amplification products; contamination of natural genomic DNA; contamination of reagents and contamination between specimens. Because once pollution occurs, the experiment will be stopped until the source of pollution is found, and the experimental results will be invalid, and the experiment needs to be re-run. Therefore, it is not only time-consuming and cumbersome to search for the source of pollution in the laboratory after pollution occurs, and it wastes manpower and material resources. Therefore, to avoid pollution, the first thing to do is to prevent, not to exclude.

The indispensable strict management of PCR laboratory

(1) Strictly control the personnel entering and leaving the laboratory. Persons unrelated to the experiment are not allowed to enter and leave the laboratory at will, and independent passages and doors to enter and exit the entire experimental area should be set up if conditions permit;

(2) Minimize unnecessary walking in the experimental area to reduce the possibility of cross-contamination.

(3) The amplification product analysis area is the main source of amplification product contamination. The waste liquid cannot be dumped in the laboratory. After being soaked in the disinfectant, it should be discarded away from the laboratory. Disposable materials such as used pipette tips are also used. It should be immersed in disinfectant and then disinfected uniformly, such as incineration;

(4) Certain substances that can cause gene mutation and toxic substances may be used in the analysis area of the amplified product, and special attention should be paid to the safety protection of the experimenter. Complete laboratory supporting facilities are a necessary condition to ensure the experimental work. Corresponding equipment and instruments should be equipped according to the different experimental contents of each laboratory, such as ultra-clean workbench, centrifuge, sampler, etc.