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product description
This product is Taq DNA Polymerase (Taq DNA Polymerase), referred to as Taq enzyme, is one of the most commonly used DNA polymerases. The PCR product amplified by this product has an A base at the 3' end and can be cloned into the T vector.
Product Specifications
Reagent composition | E001A-01(500U) | E001A-02(1000U) | E001A-03(5000U) | storage temperature |
Taq DNA Polymerase | 100μl | 200μl | 1000μl | -20℃ |
10x PCR Buffer (Mg²﹢ plus) | 1ml | 1ml*2 | 1ml*10 | |
dNTP Mixture (2.5mM each) | 100μl | 200μl | 1000μl |
Product Usage
PCR amplification of DNA
DNA sequencing
Features
Stable amplification easy to operate, stable amplification effect, and can achieve 1kb/30s extension speed.
Stable core performance: After accelerated test and room temperature storage verification, the core performance of this product is very stable.
Experimental example
Example 1 Amplification effect
Taq DNA Polymerase (E001A) was used to amplify fragments
with a length of 1kb, and the amplification results were analyzed by 1% agarose gel
electrophoresis . The amplification effect was comparable to
similar products of foreign company A. . The three on the left are Taq DNA
polymerase , and the three on the right are E001A.
Example 2 Enzyme Acceleration Experiment
Taq DNA Polymerase (E001A) was
placed 10 days to amplify fragments with a length of 1kb, and
the amplification results were analyzed by 1% agarose gel electrophoresis. The amplification
effect was similar to that of foreign A The company's similar products are comparable, and the strips are bright. The three on the left
are Taq DNA polymerase of foreign company A, and the three on the right are
E001A .
Example 3 Large fragments can be amplified without non-specific amplification
. SANSHIBIO Taq DNA Polymerase (E001A
) was used to amplify fragments with a length of 3 kb, and the amplification results were analyzed by 1%
agarose gel electrophoresis. The experimental results show that the
amplification effect of SANSHIBIO Taq DNA Polymerase is equivalent to
the similar products of domestic company B and foreign company C, and there is no non
-specific amplification. The three on the left are E001A, the three in the middle are Taq DNA polymerase from domestic
company , and the three on the right are Taq
DNA polymerase from foreign company C.
Location: Science and Technology Center, High-tech Zone, Shijiazhuang City, Hebei Province
Business Hotline: 186-3213-6937
Headquarters Tel: 0311-82970259
Email: sanshibio@126.com