Medical application of real-time fluorescence quantitative PCR instrument

⑴ Pathogen detection

At present, fluorescent quantitative PCR detection technology can detect gonococcus, Chlamydia trachomatis, Ureaplasma urealyticum, human papilloma virus, herpes simplex virus, human immunodeficiency virus, hepatitis virus, influenza virus, Mycobacterium tuberculosis, Epstein-Barr virus and cytomegalo Quantitative determination of pathogens such as viruses. Compared with the traditional detection method, it has the advantages of high sensitivity, less sampling, quick and easy and so on.

⑵ prenatal diagnosis

So far, there is no cure for hereditary diseases caused by changes in hereditary material. Only prenatal monitoring can be used to reduce the birth of sick babies to prevent the occurrence of various hereditary diseases. For example, to reduce the number of children with X-linked genetic diseases It is a non-invasive method that is easy to be accepted by pregnant women.

⑶ drug efficacy assessment

Quantitative analysis of hepatitis B virus (HBV) and hepatitis C virus (HCV) showed the relationship between the amount of virus and the curative effect of some drugs. The high level of HCV expression is insensitive to interferon treatment, while the low titer of HCV is sensitive to interferon action; during the course of lamivudine treatment, the serum level of HBV-DNA once decreased, and then increased again or exceeded the previous level. , indicating that the virus has mutated. For example, the application and significance of PCR technology in HBV detection:

a. Know the amount of hepatitis B virus present in the body.

b. Whether to copy.

c. Whether it is contagious, and how contagious it is.

d. Whether it is necessary to take medicine.

e. Whether abnormal changes in liver function are caused by viruses.

f. Determine which type of antiviral drug is suitable for the patient.

g. To determine the efficacy of drug treatment.

⑷ Tumor gene detection

Although the mechanism of tumor pathogenesis remains unclear, it is widely accepted that mutations in related genes are the underlying cause of oncogenic transformation. Increased expression and mutation of oncogenes can appear early in many tumors. Real-time fluorescence quantitative PCR can not only effectively detect gene mutations, but also accurately detect the expression of oncogenes. At present, this method has been used to detect the expression of telomerase hTERT gene, chronic myeloid leukemia WT1 gene, tumor ER gene, prostate cancer PSM gene, tumor-related virus gene and other genes. With the continuous discovery of new genes related to tumors, real-time PCR technology will play a greater role in tumor research.